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pcdna myc dbc1  (Addgene inc)


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    Structured Review

    Addgene inc pcdna myc dbc1
    Pcdna Myc Dbc1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna+myc+dbc1+plasmid/pcDNA+Myc+DBC1+(Plasmid+%2335096)/pmc12774657-170-47-56
    Average 93 stars, based on 11 article reviews
    pcdna myc dbc1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Disrupted protein interaction dynamics in a genetic neurodevelopmental disorder revealed by structural bioinformatics and genetic code expansion
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME‐NR2F2 was a gift from Nathan Lawson (Addgene plasmid #138359; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1‐hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4‐Azi was a kind gift from Irene Coin (Addgene plasmid #105829; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..

    Article Title: Structural analysis and genetic code expansion reveal the functional impact of NR2F1 mutations associated with BBSOA-Syndrome
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME-NR2F2 was a gift from Nathan Lawson (Addgene plasmid # 138359 ; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid # 135411 ; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4-Azi was a kind gift from Irene Coin (Addgene plasmid # 105829 ; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..

    Plasmid Preparation:

    Article Title: Disrupted protein interaction dynamics in a genetic neurodevelopmental disorder revealed by structural bioinformatics and genetic code expansion
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME‐NR2F2 was a gift from Nathan Lawson (Addgene plasmid #138359; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1‐hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4‐Azi was a kind gift from Irene Coin (Addgene plasmid #105829; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..

    Article Title: Deleted in Breast Cancer 1 (DBC1) is a Suppressor of B cell Activation by Negatively Regulating Alternative NF-κB Transcriptional Activity
    Article Snippet: .. Plasmids, Antibodies and Reagents PcDNA-Myc-DBC1 plasmid was purchased from Addgene ( 27 ). .. Antibodies used for immunoblotting and co-immunoprecipitation were from Bethyl laboratories (anti-p30/DBC1), Santa Cruz (anti-RelB(C-19) (D-4), RelA (F-6), C-Rel (B-6), p105/p50 (H-119), p100/p52 (C-5), and Myc (A-14), (9E10)), Sigma (anti-Flag (F7425) (F1804)) and Calbiochem (anti-Tubulin (DM1A)).

    Article Title: Structural analysis and genetic code expansion reveal the functional impact of NR2F1 mutations associated with BBSOA-Syndrome
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME-NR2F2 was a gift from Nathan Lawson (Addgene plasmid # 138359 ; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid # 135411 ; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4-Azi was a kind gift from Irene Coin (Addgene plasmid # 105829 ; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..

    Article Title: Deleted in Breast Cancer 1 (DBC1) suppresses B cell activation through RelB and is regulated by IKKα phosphorylation
    Article Snippet: .. Plasmids, Antibodies and Reagents PcDNA-Myc-DBC1 plasmid was purchased from Addgene. .. Truncation mutants were subcloned into pCMV-Myc (Clontech), and Myc-DBC1-SA mutant was generated by site directed mutagenesis using Advantage GC Rich PCR kit (Clontech) using standard protocol.

    Article Title: Deleted in Breast Cancer 1 (DBC1) suppresses B cell activation through RelB and is regulated by IKKα phosphorylation
    Article Snippet: .. PcDNA-Myc-DBC1 plasmid was purchased from Addgene. .. Truncation mutants were subcloned into pCMV-Myc (Clontech), and Myc-DBC1-SA mutant was generated by site directed mutagenesis using Advantage GC Rich PCR kit (Clontech) using standard protocol.

    Article Title: Deleted in Breast Cancer 1 (DBC1) is a Suppressor of B cell Activation by Negatively Regulating Alternative NF-κB Transcriptional Activity
    Article Snippet: .. PcDNA-Myc-DBC1 plasmid was purchased from Addgene ( 27 ). .. Antibodies used for immunoblotting and co-immunoprecipitation were from Bethyl laboratories (anti-p30/DBC1), Santa Cruz (anti-RelB(C-19) (D-4), RelA (F-6), C-Rel (B-6), p105/p50 (H-119), p100/p52 (C-5), and Myc (A-14), (9E10)), Sigma (anti-Flag (F7425) (F1804)) and Calbiochem (anti-Tubulin (DM1A)).

    Ligation:

    Article Title: Disrupted protein interaction dynamics in a genetic neurodevelopmental disorder revealed by structural bioinformatics and genetic code expansion
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME‐NR2F2 was a gift from Nathan Lawson (Addgene plasmid #138359; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1‐hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4‐Azi was a kind gift from Irene Coin (Addgene plasmid #105829; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..

    Article Title: Structural analysis and genetic code expansion reveal the functional impact of NR2F1 mutations associated with BBSOA-Syndrome
    Article Snippet: .. The genes were then excised and cloned to replace DBC1 in the pcDNA Myc DBC1 plasmid by restriction ligation at the EcoRI/XhoI restriction sites. pME-NR2F2 was a gift from Nathan Lawson (Addgene plasmid # 138359 ; http://n2t.net/addgene:138359 ; RRID:Addgene_138359). pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096 ; RRID:Addgene_35096). pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid # 135411 ; http://n2t.net/addgene:135411 ; RRID:Addgene_135411). pIRE4-Azi was a kind gift from Irene Coin (Addgene plasmid # 105829 ; http://n2t.net/addgene:105829 ; RRID:Addgene_105829). ..



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